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. 2019 Oct 21;10(26):6543–6556. doi: 10.7150/jca.34285

Figure 1.

Figure 1

Growth inhibition and apoptosis induction in A549 and H1299 cells by EGCG. Growth inhibition curves A549 (A) and H1299 (B) cells exposed to various concentrations (20, 80 and 160 μM) of EGCG for 48 and 72 h, as determined using an MTT assay. Each value is expressed as the mean ± SD (n=3). (C) The effects of EGCG on colony formation in H1299 and A549 cells. (D) Quantification of colony formation assay. (E) Flow cytometry images. (F-G) Quantitative analysis of the percentage of apoptotic cells of EGCG after a 48 h incubation. The percentage of total apoptotic cells was defined as the sum of early and late apoptotic cells. (H-J) Western blot analysis of Caspase 3 (C3), cleaved Caspase 3 (CC3), Caspase 9 (C9), cleaved Caspase 9 (CC9), Bcl-2 and β-actin expression in A549 and H1299 exposed to different concentrations of EGCG.