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. 2019 Oct 17;158(4):340–352. doi: 10.1111/imm.13121

Figure 6.

Figure 6

Siglec‐F knockdown enhances signal transducer and activator of transcription 5 (STAT5) phosphorylation induced by granulocyte–macrophage colony‐stimulating factor (GM‐CSF) in macrophage colony‐stimulating factor‐differentiated bone‐marrow‐derived macrophages (M‐BMDMs). (a) Schematic presentation of the knockdown experiment. M‐BMDMs were transfected with Siglec‐F or control small interfering RNA (siRNA). Cells were washed after a 48‐hr culture and stimulated with GM‐CSF for the indicated periods. Phosphorylation was assessed by Western blotting. (b) Siglec‐F knockdown enhanced the phosphorylation of STAT5. Total and the phosphorylated form of STAT5 were examined. Actin was measured as a control. A representative result of three independent experiments is shown. (c) Quantification of band intensity. The band intensities of pSTAT5 were normalized to that of actin. The band intensity of control siRNA at 15 min was regarded as 1. White and black bars indicate control and Siglec‐F siRNAs, respectively. Data are the mean ± SE of three independent experiments. *P < 0·05 versus the control at the same time point by Student's t‐test.