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. 2019 Nov 7;34(9):597–603. doi: 10.1089/cbr.2019.3049

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Copyright 2019, Mary Ann Liebert, Inc., publishers

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FIG. 2. — Fluorescence staining of MC1Rs on B16/F10 melanoma cells (A) using MC1R antibody (white). The nuclei were stained with DAPI (grey). The microscopic images were acquired by confocal laser microscopy at 100 × magnification. Scale bar, 20 μm. Radioactive HPLC profile of ⁹⁰Y-DOTA-GGNle-CycMSH_hex (B, T_R = 19.3 min) and its mouse serum stability (C) after 4 h incubation at 37°C. Arrows indicate the original compound of ⁹⁰Y-DOTA-GGNle-CycMSH_hex. Specific binding of ⁹⁰Y-DOTA-GGNle-CycMSH_hex on B16/F10 (D) cells with or without peptide blockade. DAPI, 4′,6-diamidino-2-phenylindole; HPLC, high-performance liquid chromatography; MC1R, melanocortin-1 receptor.