Table 1.
Distribution of distinct populations of innate immune cells T- and B- lymphocytes in the CD4null patient here reported compared to age-matched reference values.
| Leucocyte subsets | Patient | Age reference values |
|---|---|---|
| T-cells | 1,700 ± 733 | (743–2,379) |
| CD4+ CD8− TCRγδ− T-cells | 0 ± 0 | (501–1,654) |
| Naïve | 0 ± 0 | (83–1,057) |
| Central memory/transitional memory | 0 ± 0 | (235–784) |
| Effector memory | 0 ± 0 | (25–208) |
| Terminally differentiated | 0 ± 0 | (0–663) |
| CD4− CD8+ TCRγδ− T-cells | 902 ± 367 | (133–1,432) |
| Naïve | 556 ± 226 | (29–386) |
| Central memory/transitional memory | 217 ± 98 | (59–453) |
| Effector memory CD27− | 105 ± 26 | (6–323) |
| Effector CD27dim | 4 ± 6 | (7–457) |
| Terminally differentiated | 33 ± 14 | (0–500) |
| TCRγδ+ T-cells | 238 ± 96 | (7–231) |
| CD4− CD8− TCRγδ− T-cells | 560 ± 287 | (4–24) |
| Treg-like (CD8−/TCRγδ−/CD25++/CD127−) | 66 | (22–141)* |
| TFH-like (CD8−/TCRγδ−/CXCR5+) | 134 | (45–240)* |
| Th1-like (CD8−/TCRγδ−/CXCR3+/CCR4−/CCR6−/CXCR5−) | 139 | (57–704)* |
| Th2-like (CD8−/TCRγδ−/CXCR3−/CCR4+/CCR6−/CXCR5−) | 55 | (24–123)* |
| Th17-like (CD8−/TCRγδ−/CXCR3−/CCR4+/CCR6+/CXCR5−) | 43 | (14–93)* |
| Th1/Th17-like (CD8−/TCRγδ−/CXCR3+/CCR4−/CCR6+/CXCR5−) | 146 | (20–124)* |
| NK-cells | 603 ± 134 | (150–672) |
| Classical monocytes | 610 ± 258 | (343–1,104) |
| CD62L+ cMo | 249 | (2–731)* |
| CD62L− cMo | 220 | (19–473) |
| Non-classical monocytes (CD16++) | 169 ± 102 | (26–141) |
| iMo (CD14+/CD16++) | 21 | (0–89) |
| Late ncMo (CD14−/CD16++) | 120 | (0–160) |
| SLAN− Late ncMo | 96 | (0–155) |
| SLAN+ Late ncMo | 24 | (0–52) |
| Plasmacytoid DCs | 6 ± 3 | (4–29) |
| Neutrophils | 3,872 ± 787 | (1,800–6,782) |
| Eosinophils | 213 ± 64 | (0–648) |
| Basophils | 62 ± 15 | (10–64) |
| B-cells | 517 ± 241 | (48–413) |
| Immature B-cells | 7.5 ± 2.1 | (0.8–23) |
| Naïve B-cells | 394 ± 155 | (26–244) |
| CD21+ | 388 ± 151 | (24–372) |
| CD21− | 5.5 ± 3.5 | (0.3–31) |
| Memory B-cells | 209 ± 77 | (25–173) |
| CD27+ | 194 ± 71 | (19–160) |
| CD27− | 15 ± 5.7 | (1.4–17) |
| CD21+ | 201 ± 76 | (16–144) |
| CD21− | 8.0 ± 1.4 | (2.8–33) |
| IgM++D+ | 82 ± 38 | (12–114) |
| IgG1+ | 42 ± 11 | (2.8–30) |
| IgG2+ | 17 ± 3.5 | (0.6–14) |
| IgG3+ | 9.0 ± 4.2 | (0.7–6) |
| IgG4+ | 0.8 | (<0.01–4.1) |
| IgA1+ | 44 ± 18 | (2.5–27) |
| IgA2+ | 13 ± 1.4 | (0.4–14) |
| Only IgD+ | 0.7 ± 1.0 | (<0.01–0.9) |
| Plasmablasts | 6.6 ± 6.2 | (0.6–9.7) |
| IgM+ | 0.07 | (0.04–1) |
| IgG1+ | 0.3 | (<0.01–1.7) |
| IgG2+ | <0.01 | (<0.01–0.7) |
| IgG3+ | 0.08 | (<0.01–0.2) |
| IgG4+ | <0.01 | (<0.01–0.1) |
| IgA1+ | 6.2 | (0.2–3.8) |
| IgA2+ | 0.4 | (0.04–2.9) |
| Only IgD+ | <0.01 | (<0.01–0.1) |
Results expressed as (mean ± standard deviation) absolute cell counts per μl of peripheral blood, obtained from repeated analysis performed during the last 5 years following diagnosis. Normal reference values expressed as minimum and maximum of age-matched healthy donors (40–59 years) based on previously published data (20, 21, 26, 28) are also shown between brackets (). Populations that showed abnormal absolute numbers compared to age-reference values are depicted in bold.
Reference values obtained from CD4+ CD8− TCRγδ− T-cells of age-matched healthy donors. DNT, double-negative T-cell; Tregs, regulatory T-cells; TFH, follicular helper T-cells; DCs, dendritic cells; cMo, classical monocytes; iMo, intermediate monocytes; ncMo, non-classical monocytes.