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Quantification method using newCAST™ Visiopharm® software. Representative images showing super image capture of a biopsy sample (A); manual outlining of the region of interest (ROI) excluding non-clinically relevant areas, indicated by the green dotted line (B); unbiased random sampling (C); detailed cell quantification at 400x magnification (D). Double-immunofluorescence staining for CD68 and pSTAT1 (E) or cMAF (F).
