Table 2.

Antibodies used for immunohistochemical investigation of canine Leydig cell tumors.

Antibody against:	Source	Type/clone	Dilution	Pretreatment
Inhibin-alpha	Bio-Rad AbD Serotec, Kidlington, UK	mAb/R1	1:50	Citrate buffer, pH 6.0, HIER, 10 min
Melan A	Leica Biosystems, Newcastle, UK	mAb/A103	1:50	EDTA buffer, pH 9.0, HIER, 10 min
Ki-67	Nichirei Biosciences, Tokyo, Japan	mAb/SP6	Ready-to-use	EDTA buffer, pH 9.0, HIER, 20 min

EDTA = ethylenediamine tetra-acetic acid; HIER = heat-induced epitope retrieval; mAb = monoclonal antibody.