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. 2019 Oct 22;9(26):8409–8425. doi: 10.7150/thno.36605

Figure 5.

Figure 5

miR-302a inhibits NFIB/ITGA6 axis-mediated migration and invasion in CRC cells. A, B Representative images and graph of NFIB downregulation in DLD-1 cells, as determined by Transwell migration and invasion assays (A) and wound-healing assay (B). Scale bar, 500 μm. C, D Representative images and graph of NFIB down- or upregulation in Caco-2, as determined by Transwell migration and invasion assays (C) and wound-healing assay (D). Scale bar, 500 μm. E, F Representative images and graph of miR-302a overexpression in combination with or without NFIB upregulation, as determined by Transwell migration and invasion assays (E) and wound-healing assay (F). Scale bar, 500 μm. G Western blot analyses of ITGA6 expression with NFIB up- or downregulation in the indicated cells. H Schematic presentation of NFIB binding sites in the ITGA6 promoter region (left) and gel electrophoresis image of the ChIP analysis (right). I, J Representative images and graph of ITGA6 downregulation in DLD-1 cells, as determined by Transwell migration and invasion assays (I) and wound-healing assay (J). Scale bar, 500 μm. The values represent the mean ± SEM. *P<0.05, **P<0.01, Student's t test.