GINS4 promotes cell proliferation, cell cycle, migration and invasion, and suppresses cell apoptosis in vitro. Stable MGC-803 cells with GINS4 knockdown and stable AGS cells with GINS4 overexpression were constructed. A&B, GINS4 mRNA and protein expression in stable MGC-803 and AGS cells were detected. C, Cell counting of MGC-803 and AGS cells with different GINS4 levels. D, MTT assays of MGC-803 and AGS cells with different GINS4 levels. E&F, Flow cytometric analysis of the cell cycle in MGC-803 and AGS cells. G&H, Flow cytometric analysis of cell apoptosis in MGC-803 and AGS cells. I&J, Cell wound healing assays of MGC-803 and AGS cells with different levels of GINS4. K&L, Transwell migration and invasion assays of MGC-803 and AGS cells with different levels of GINS4. All data are presented as the mean ± SEM of three experiments. *P<0.05, **P< 0.01.