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. 2019 Oct 21;9(26):8294–8311. doi: 10.7150/thno.36256

Figure 6.

Figure 6

MiR-509-3-5p suppresses GINS4 expression by directly binding to the GINS4 mRNA 3'UTR. A&B, MiR-509-3-5p expression in 61 fresh frozen gastric cancer tissues. C, The correlation between GINS4 mRNA expression and miR-509-3-5p expression in 61 fresh frozen gastric cancer tissues. D, The ISH results showed that the level of miR-509-3-5p was lower in gastric cancer tissues than that in matched adjacent normal tissues of the gastric cancer TMA. E&F, The correlation between GINS4 protein expression and miR-509-3-5p expression in normal gastric tissues and gastric cancer tissues of the TMA. G, GINS4 mRNA expression was detected after transfection with miR-509-3-5p/mimics or miR-509-3-5p/inhibitor. H, The effects of altered miR-509-3-5p expression on the level of GINS4 protein and the activation of Rac1/CDC4 and their downstream pathways were detected. I&J, The WT or mutant GINS4 mRNA 3'UTR was respectively cloned into luciferase reporter plasmids. The relative luciferase activity in MGC-803 (I) and AGS cells (J) is shown. All data are presented as the mean ± SEM of three experiments. *P<0.05, **P< 0.01.