Figure 8.

(a) Schematic of CDNP preparation by lysine crosslinking of succinyl- β-cyclodextrin (orange) and subsequent drug loading by guest-host complexation of R848 (blue). (b) Schematic overview of the tumor microenvironment, where TAMs were mainly canonically M2-like; however, their behavior was pharmacologically influenced. (c) Gene expression of M2-like (IL-4 treated) and M1-like (LPS/INF-γ treated) murine macrophages. (d) Raw images were processed by automated segmentation for the measurement of prominent features useful in the identification of M1-like polarization as indicated in yellow, where the mean radius (solid line), minor axis length (dotted line) and perimeter (dashed line). Cells were stained for nuclei (DAPI, red), actin (phalloidin, green), and cell membrane (WGA, blue). Scale bar, 25 μm. Adapted with permission from ¹¹⁴, copyright 2018 Nature Publishing Group.