Figure 1.

Pten loss in Lgr5⁺ HFSCs induces HF hyperplasia. Lgr5-CreER;Pten^flox/flox mice aged 3 weeks were treated with or without tamoxifen (TAM), and the skin tissues were harvested for histological analysis at different time points. (A) 20 days after TAM treatment, Lgr5-Pten^-/-mice showed enlargement of the HF with increased cell number (hyperplasia). (B) 40 days after TAM treatment, in Lgr5-Pten^-/-mice the HF entered telogen phase, but they were still larger than telogen HFs in Lgr5-Pten^+/+ mice.100 hair follicles per mouse were measured, 10 mice were analyzed. Scale bars, 100 μm. Epi, Epidermis; Derm, Dermis; HF, hair follicle. Data are expressed as the mean±s.e.m. ***P< 0.005.