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. 2019 Sep 4;198(3):416–429. doi: 10.1111/cei.13365

Figure 2.

Figure 2

The cell surface expression of tumor necrosis factor (TNF) receptor type I (TNF‐R1) is impaired with the G87V TNFRSF1A mutation. HEK293 cells were transiently transfected with the indicated wild‐type (WT) or mutant TNF‐R1‐green fluorescent protein (GFP) fusion constructs. The cells were stained with the fluorochrome‐labeled anti‐TNF‐R1 antibody. Cell surface or intracellular expression of TNF‐R1 was determined by flow cytometry. The cells were gated based on the GFP‐positive cells, and then histograms representing TNF‐R1 expression were generated. T79M (c.236 C>T, p.Thr79Met) and R121Q (c.362 G>A, p.Arg121Gln) were used as controls. T79M and R121Q mutations are known to be related to severe and mild phenotypes of TRAPS, respectively. Data are representative of three independent experiments.