Figure 3.

Nuclear factor‐kappa B (NF‐κB) promotor activity in HEK293 cells transfected with TNFRSF1A variants. HEK293 cells were transfected with wild‐type (WT) or mutant tumor necrosis factor receptor 1 (TNF‐R1)‐expression constructs together with an NF‐κB luciferase reporter vector and a secreted alkaline phosphatase (SEAP) expression vector, as a transfection efficiency control. After incubation for 24 h, the activity of each reporter enzyme was measured to calculate the relative NF‐κB transcriptional activity in each sample. The value in cells expressing WT TNF‐R1 is shown as 100%. Data represent the average of three independent experiments. Values are presented as means ± standard deviation (s.d.). *P < 0·05 between WT and indicated mutant TNF‐R1 constructs.