Figure 4.

Mitochondrial reactive oxygen species (ROS) production in peripheral blood mononuclear cells (PBMCs) of tumor necrosis factor (TNF) receptor‐associated periodic syndrome (TRAPS) patients. PBMCs were isolated from healthy controls (HC; 16 PMBC samples from 12 individuals) and TRAPS patients (eight PBMC samples from two individuals) by density gradient separation. PBMCs were incubated with MitoSOX Red indicator for 10 min, and then ROS levels were determined by flow cytometry. Cells were gated based on monocytes (a,b) and lymphocytes (c,d) according to forward‐ and side‐scatter characteristics. Representative results of mitochondrial ROS production are shown in histograms (a,c). Data were normalized to the average values of healthy controls in each experiment. *P < 0·05.