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. 2019 Nov 8;10:1287. doi: 10.3389/fphar.2019.01287

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Copyright © 2019 Wu, Wang, Lin, Shang, Wang, Zhou, Wang, Zhang, Luo and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CuC induces cell apoptosis. (A) Apoptosis assay of cancer cells with CuC treatment by annexin V-FITC and PI staining. CuC induced significant early apoptosis at 100 mM and 1 µM treatment in T24 and HepG2 cells (**, p < 0.01). (B) Western blot (WB) analysis of indicated apoptotic markers after 48 h dosage of serial CuC in T24 and PC-3 cells.