Table 3.
Genotypic and phenotypic profiles of 10 MDR P. aeruginosa isolates that were found to be non-susceptible to ceftazidime/avibactam, ceftolozane/tazobactam or both
| Sample number |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PA9 | PA37 | PA99 | PA11 | PA12 | PA98 | PA123 | PA125 | PA199 | PA154 | |
| ST (serogroup) | 235 (O11) | 235 (O11) | 235 (O11) | 308 (O11) | 308 (O11) | 308 (O11) | 292 (O12) | 823 (O11) | 233 (O6) | 27 (O1) |
| β-Lactamase gene (molecular class), gene presence (% identitiy) | ||||||||||
| TEM-116 (class A) | − | − | − | − | − | − | − | − | − | yes (100) |
| VEB-1a (class A) | − | − | yes (100) | yes (100) | yes (100) | yes (100) | − | − | − | − |
| CARB-3 (class A) | − | − | − | − | − | − | yes (99.67) | − | − | − |
| VIM-2 (class B) | yes (100) | yes (100) | − | − | − | − | − | yes (100) | yes (100) | − |
| PDC-2 (class C) | yes (99.75) | yes (99.75) | yes (99.75) | − | − | − | − | − | − | − |
| PDC-3 (class C) | − | − | − | − | − | − | − | − | yes (100) | − |
| PDC-5 (class C) | − | − | − | − | − | − | yes (99.75) | − | − | yes (99.75) |
| PDC-7 (class C) | − | − | − | yes (99.75) | yes (99.75) | yes (99.75) | − | yes (98.99) | − | − |
| OXA-4 (class D) | − | − | − | − | − | − | − | − | yes (100) | − |
| OXA-10 (class D) | − | yes (99.62) | yes (100) | − | − | − | − | − | − | − |
| OXA-50 (class D) | yes (99.24) | yes (99.24) | yes (99.24) | yes (99.24) | yes (99.24) | yes (99.24) | yes (98.09) | yes (98.33) | yes (99.24) | yes (99.24) |
| Efflux pump regulators | ||||||||||
| MexR | + | + | + | + | + | + | + | + | + | + |
| NalC | + | + | + | + | + | + | + | + | + | + |
| NalD | + | + | + | + | + | + | + | + | + | + |
| CpxR | + | + | + | − | + | + | − | + | + | + |
| SoxR | + | + | + | − | + | + | − | − | + | + |
| type B NfxB | + | + | + | − | + | + | − | − | + | + |
| Efflux pump complex | ||||||||||
| MexAB-OprM | + | + | + | − | + | + | + | + | + | + |
| MexCD-OprJ | + | + | + | + | + | + | + | + | + | + |
| MexPQ-OpmE | + | + | + | + | + | + | + | + | + | + |
| MuxABC-OpmB | + | + | + | + | + | + | + | + | + | + |
| Antimicrobial susceptibility result (MIC in mg/L) | ||||||||||
| CZA | NS (128) | NS (96) | NS (12) | NS (24) | NS (16) | S (2) | S (1.5) | NS (24) | NS (32) | S (8) |
| C/T | NS (256) | NS (256) | NS (256) | NS (256) | NS (256) | NS (256) | NS (50) | NS (256) | NS (256) | NS (12) |
| MEM | NS (32) | NS (32) | NS (32) | NS (32) | S (2) | S (1.5) | S (0.75) | NS (32) | NS (32) | NS (32) |
| Mucoidity | non-mucoid | non-mucoid | non-mucoid | mucoid | non-mucoid | mucoid | non-mucoid | non-mucoid | mucoid | non-mucoid |
CZA, ceftazidime/avibactam; C/T, ceftolozane/tazobactam; MEM, meropenem; NS, non-susceptible; S, susceptible; +, present; −, absent.