Table 4.
The processing and storage of biomaterials in the OncoLifeS data-biobank
| Serum | 10 mL serum clot tube (BD 367896) | Centrifuge (10 min, 1300g, RT)—aliquot serum 5×, store at − 80 °C |
| EDTA-plasma + buffy coat (DNA) | 10 mL K2-EDTA tube (BD 367525) | Centrifuge (10 min, 1300g, RT), aliquot buffy coat 1×, aliquot EDTA-plasma 5× store at − 80 °C |
| Heparin-plasma |
10 mL Li-heparin tube (BD 367526) 2 × 10 mL Li-heparin tube (BD 367526) |
Centrifuge (10 min, 1300g, RT), aliquot heparin-plasma 5× store at − 80 °C Monoculear cell isolation over lymphoprep, 100e6 MNCs per vial, stored in liquid nitrogen |
| Plasma (for cell-free DNA) | 2 × 8 mL tube (Streck 218997) | Centrifuge (20 min, 1600g, RT), collect plasma, centrifuge (10 min, 16,000g, RT), aliquot cell-free DNA plasma 10×, store at − 80 °C |
| Whole blood (for DNA) | 10 mL K2-EDTA tube (BD 367525) | No processing, store tube at − 80 °C |
| Whole blood (for RNA) | Paxgene tube (BD 762165) | No processing, store tube at − 80 °C |
| Bone marrow | 3 × 10 mL syringe with Na-heparin | Isolation of mononuclear cells by density centrifugation, controlled freezing of isolated mononuclear cells, store in liquid nitrogen |
| Feces swab in glycerol b | Glycerol swab (Copan Custom kit M1016004) | No processing, store tube with swab at − 80 °C |
| Feces | 3 × 2.0 mL cryotubes (Greiner 122280) | No processing, store cryotubes at − 80 °C |
| Urine | 9.5 mL urine tube (BD 365000) | Centrifuge (10 min, 1300g RT), aliquot urine 6×, store at − 80 °C |
| Tumor tissue (for paraffin embedding) | Tissue fixed in formalin | Tissue embedded in paraffin, store at RT |
| Tissue adjacent to a tumor (for frozen section) | Tissue in tin | Tissue in tin, store at − 80 °C |
| Tissue adjacent to a tumor (for paraffin embedding) | Tissue fixed in formalin | Tissue embedded in paraffin, store at RT |
| Healthy tissue (for frozen section) | Tissue in tin | Tissue in tin, store at − 80 °C |
RT room temperature