Fig. 2.

Parabiosis experiment indicating no recruitment of circulating LysM^GFP myeloid cells to the hypothalamus during 12-week HFD feeding. a, b Representative images of parabiosis between LysM^GFP and C57 mice and FACS analysis data confirming successful parabiosis. The percentages indicate the proportion of LysM^GFP cells among the peripheral blood monocytes. c Confocal images of GFP and DAPI immunostaining in the choroid plexus and hypothalamus of LysM^GFP and C57 parabionts showing that circulating LysM^GFP cells had infiltrated the choroid plexus but not the hypothalamus of the C57 mice. Scale bars, 100 μm. ARC, hypothalamic arcuate nucleus; D3V, dorsal third ventricle; ME, median eminence; 3V, third ventricle