Fig. 1.

Spontaneous accumulation of SNVs in isogenic cell clones. a Experimental scheme. The experiments were started with an ancestral single cell clone. After 50 days of culturing, a further cloning step was performed. Genomic DNA was extracted from each ancestral and descendent clone as soon as a sufficient number of cells were available. b The mean number of newly arising SNVs detected per sequenced genome in each indicated cell line. Red symbols show the values for individual samples, error bars indicate standard error of the mean (SEM). c Mean spontaneous triplet SNV mutation spectrum in the indicated cell lines. Each mutation class, as indicated at the top of the panel, is separated into 16 categories based on the identity of the preceding and following nucleotide as shown below. The order of the following nucleotides, not shown due to lack of space, is alphabetical