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. 2019 Nov 14;19:255. doi: 10.1186/s12866-019-1633-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — L. monocytogenes HtrA is active under stress conditions. a The activity of LmHtrA expressed by L. monocytogenes EGD grown to the logarithmic phase or stationary phase was detected in 50 μg whole cell lysates by casein zymography (left panel). 10 μg whole cell lysate of L. monocytogenes EGD in different growth phases were analyzed for HtrA expression by Western blotting using a specific anti-LmHtrA antibody (right panel). The zymogram has been repeated four times with lysates from independent experiment and the HtrA detection by Western blot has been performed by two independent experiments. b Recombinant LmHtrA was incubated at indicated temperatures for 30 min and the activity of HtrA multimers, monomers and truncated HtrA (short HtrA, sHtrA) was investigated in casein zymography. This experiment has been repeated three times