Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2019 Nov 15.

Published in final edited form as: Biochimie. 2019 Jul 24;165:123–130. doi: 10.1016/j.biochi.2019.07.016

Fig. 2. — HEK293T KO TMEM165 cells were transfected with empty-vector, wild-type or TMEM165 constructs. Total cells lysates were obtained, subjected to SDS-PAGE, Western blot was performed with the respective antibodies. A. LAMP2 and TMEM165 profiles obtained 24 h after transfection. B. Ratio of fully glysosylated forms of LAMP2 (percentage of fully glysosylated forms versus the total LAMP2). C. Immunofluorescence analysis of the expression and localization of TMEM165 in transfected cells with mutated forms of TMEM165 in conserved amino-acids. (GM130 = Golgi marker) D. Illustration of red and green fluorescence merge with RGB Profiler (ImageJ Fiji®).

Fig. 2. — HEK293T KO TMEM165 cells were transfected with empty-vector, wild-type or TMEM165 constructs. Total cells lysates were obtained, subjected to SDS-PAGE, Western blot was performed with the respective antibodies. A. LAMP2 and TMEM165 profiles obtained 24 h after transfection. B. Ratio of fully glysosylated forms of LAMP2 (percentage of fully glysosylated forms versus the total LAMP2). C. Immunofluorescence analysis of the expression and localization of TMEM165 in transfected cells with mutated forms of TMEM165 in conserved amino-acids. (GM130 = Golgi marker) D. Illustration of red and green fluorescence merge with RGB Profiler (ImageJ Fiji®).