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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Exp Parasitol. 2019 May 28;203:8–18. doi: 10.1016/j.exppara.2019.05.007

Figure 2. Enzymatic activity and cellular localization of TgNMT.

Figure 2.

(A) TgNMTp was incubated with either with a standard substrate (pp60src) or a putative T. gondii substrate (TgAMPKb; access number Toxodb: TGME49_268960) as described in the Material and Methods section. Activity is expressed as a percentage of the relative fluorescence units (RFU) obtained with pp60src. Appropriate blanks (without Myristoyl-CoA or substrate) are already subtracted. (B) Indirect Immunofluorescence assay using the anti-TgNMT serum (NMT, red) on intra- (I) or extracellular (E) parasites. Mouse anti-SAG1 (SAG1, green) was used to label the plasma membrane and DAPI was used to mark nuclei. Parasites were observed by phase-contrast microscopy (PC).