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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: J Comp Neurol. 2018 Dec 24;527(6):1056–1069. doi: 10.1002/cne.24588

Figure 4.

Figure 4.

(a,b) Co-localization of CRFR1-GFP and ERα. Dual-label fluorescent immunohistochemistry showing that females have significantly more CRFR1-GFP, ERα, CRFR1-GFP/ERα co-localized, and a higher percentage of CRFR1-GFP cells that co-express ERα compared to males. (c-f) Representative images from a female AVPV/PeN showing a low magnification image of co-localized cells (c), CRFR1-GFP (d; green), ERα (e; magenta), and coexpression of CRFR1/ERα (f; white). (g) Image showing co-expression of CRFR1 and ERα on the day of birth (P0) in the female mouse AVPV/PeN. Inset box indicates region that was further magnified. Arrows indicate examples of co-localized cells. * Indicates statistical significance (p≤0.05), data are reported as mean ± SEM. N=5 per sex. 3V, third ventricle.