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Adult gonadectomy effects on CRFR1-GFP+ cells. (a) Gonadectomy did not alter CRFR1-GFP+ cell number in either males or females. Representative images from female (b) and male (d) sham surgery compared with female (c) and male (e) gonadectomy, showing that removal of adult gonadal hormones does not change the expression pattern. * Indicates significant sex difference (p≤0.001), data are reported as mean ± SEM. N=8 per sex/treatment. 3V, third ventricle.
