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Nephrin/Nck1 cluster formation is independent of actin polymerization. TIRF time-lapse images of a live HeLa cell expressing Src-FKBP, Nephrin-FRB (shown in video) and Nck1. Cells were treated with 10 min of MβCD, 15 min of latrunculin B followed by rapamycin. Images were captured every 30 s after adding rapamycin to the media.
