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. 2019 Nov 15;30(24):2953–2968. doi: 10.1091/mbc.E19-05-0247

FIGURE 6:

FIGURE 6:

starPEG-(KA7)4 interferes with microtubule binding of kinesin-1 but not with its motility. (A) Kymographs showing the motility of GFP-labeled truncated rat kinesin-1 (rKin-430-GFP) on taxol-stabilized microtubule in the presence of starPEG-(KA7)4-TAMRA at 25°C and the indicated concentrations. Kinesin landing rates are given below the kymographs. Probability values are derived from a Mann–Whitney test. (B) Frequency distribution of kinesin-1 velocities in the presence of starPEG-(KA7)4-TAMRA at 0 nM (magenta), 5 nM (cyan), and 25 nM (blue). The dashed lines indicate the respective 95% confidence intervals. Numbers given are maxima of Gaussian fits ± SE. Probability values are derived from a Mann–Whitney test. (C, D) Cumulative frequency plots of kinesin-1 dwell time (C) and run length (D) in the presence of starPEG-(KA7)4-TAMRA at the indicated concentrations. Color coding of the data as in B. Numbers given are the respective half-maxima ± SE. Probability values are derived from a Mann–Whitney test.