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. 2019 Nov 15;30(24):2969–2984. doi: 10.1091/mbc.E19-05-0284

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© 2019 Liao, Tham, Liang, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 9: — The UP-positive LDs in Snx31-KO urothelium are associated with autophagy markers. (A–C) Paraffin sections of urothelia from normal (Wt) or Snx31-KO mice (as marked) were double stained using antibodies to UPIb and LC3, an autophagy marker. (D) Immunoblotting showing the appearance in the KO urothelium of a 16-kDa band (*) indicating LC3 activation (lane 2 vs. control in lane 1). Four animals from each line were used. (E) Costaining of the KO urothelium for UPIb, caspase 3, LAMP1, and DAPI showing the partial colocalization of UP and LAMP1 and induction of apoptosis. (F) TUNEL assay for the Wt (F1, F3) or Snx31-KO urothelia (F2, F4). Note the colocalization of uroplakins with markers of autophagy (B3), and the expression of apoptotic markers in some of the LD-laden cells. Scale bars = 10 µm in all IF staining panels.