TABLE 1:
Progressive changes in the size and antigenic properties of mitochondrial lipid droplets during different stages of lipid drop (LD) formation in the Snx31-KO urothelial cells.
| Properties/markersa | Figures | Normal mitochondria | Small intramitochondrial LDs (stage I labeled * in Figure 4, D–K) | Larger LDs in mitochondria with damaged or no cristae (stage II, **; Figure 4, L and M) or with cytoplasmic exposure (stage III, ***; Figure 5, A–F) | Autophagosome | Lysosome | |
|---|---|---|---|---|---|---|---|
| Size | 4, 5, 6, and 7, A–C | ∼0.5 µm | 0.5–0.8 µm | 1–5 µm | 0.5–1.5 µm | 1–2 µm | |
| Uroplakins | UPIa, II, IIIa, IIIb | 1, 6, 7, 8, 9, and 10 | – | ++ | ++ | + | + |
| Lipid | Lipophlic dyes | S1, F–H | – | ++ | ++ | ||
| PLIN1 and 2 | 7, M and N | – | ++ | ++ | |||
| Mitochondrial markers | AIF, Cox4 | 7K and 7O | ++ | ++ | ++ | ||
| Mitofusin | Unpublished data | + | ++ | ++ | |||
| PINK1, PARK2 | 7L and unpublished data | – | + | ++ | |||
| Mito-ID Green, MMP | 7, A–J | + | +++ | +++ | |||
| ER | Sec23A | 8B | – | ++ | ++ | ||
| Calnexin | 8D | – | +/– | + | |||
| Golgi | GM130 | 8E | – | – | – | ||
| TGN38 | 8F | – | + | ++ | |||
| Autophagosome | LC3 | 9, B–D | – | ++ | ++ | ++ | |
| Lysosome | LAMP1 | 1, J–L, and 9E | – | + | + | + | ++ |
aSee text for the references of the markers.