Fig. 5.

GRASP55 deacetylation is required for Golgi reassembly at mitotic exit. (A) GRASP55/65 double-knockout cells were transfected with a GFP vector (as control), or GFP-tagged WT, K50R or K50Q GRASP55 (labeled G55), and stained for GM130. Representative fluorescence images of telophase and cytokinesis cells are shown. Arrowheads indicate cells with fragmented Golgi. Indicated areas, showing the representative Golgi morphology, are enlarged in the insets. Scale bar: 20 μm. (B) Quantification of Golgi fragmentation in A from three independent experiments (n=200). Quantification results are presented as mean±s.e.m. **P<0.01; ***P<0.001 (two-tailed Student's t-test).