Fig. 6.

Acetylation-deficient GRASP55 partially rescues Golgi fragmentation caused by SIRT2 depletion. (A) HeLa cells were transfected first with SIRT2 siRNA for 48 h, then with a GFP vector (as control) or GFP-tagged WT, K50R or K50Q GRASP55 (labeled G55) for another 24 h, and stained for GM130. Indicated areas, showing the representative Golgi morphology, are enlarged in the insets. Scale bar: 20 μm. (B) Cells in A were analyzed by western blotting to show the knockdown efficiency of endogenous SIRT2 and the expression level of GFP-tagged GRASP55. (C) Quantification of Golgi fragmentation in A from three independent experiments (n=221). Quantification results are presented as mean±s.e.m. *P<0.05; ***P<0.001 (two-tailed Student's t-test).