Figure 1.

Tumor associated IL-6 and IL-8 correlate with disease stage and tumor burden. (A) Analysis of circulating cytokines from healthy donors compared to stage IV melanoma patients. (Note: IL-13 was measured to be below the limit of detection and is not displayed). (B) Circulating IL-6 or IL-8 compared between healthy donors, stage I, and stage IV melanoma patients. Dotted line denotes limit of detection. (C) Paraffin-embedded tumor samples from stage IV patients were stained for IL-6 and IL-8 by IHC. Scale bars represent 200 μM, green lines indicate regions identified by a trained pathologist as tumors. (D) Correlation between circulating IL-6 (top) or IL-8 (bottom) and the percentage of positive-staining tumor. Correlation between the circulating concentration of IL-6 (E) or IL-8 (F) vs. tumor burden. (G) Correlation of circulating IL-6 and IL-8, *p < 0.05.