Figure 4.

Localization of fluorescent chimeric proteins containing N-terminal SECE1 regions and C-terminal SECE2 regions in transfected protoplasts. (A) Diagrams depicting constructs III, IV, and V. SECE1 sequences are indicated with light gray and SECE2 sequences with dark gray; N1 and N2: N-terminal region; GFP: green fluorescent protein; Sign.: signature domain; TMD: linker plus transmembrane domain; Tail: C-terminal tail. (B–D) Leaf protoplasts from 4–5 week old wildtype (Columbia ecotype) plants were transfected with (B) construct III, (C) construct IV, or (D) construct V. The images show GFP fluorescence (cyan), chlorophyll fluorescence (magenta), merged images, and relative pixel intensity diagrams that correspond with the white lines on the merged images.