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. 2019 Nov 8;10:1326. doi: 10.3389/fpls.2019.01326

Table 2.

Biological mutagens applied for induction of random mutations in rice.

Method Mechanism of mutation Mutation frequency Mutants produced Pros Cons
T-DNA* Random sequence insertion 88.8% (6645 insertions of 7480) (Sallaud et al., 2004) Plant development and metabolism (Basu et al., 2017; Chen et al., 2017a; Hinrichs et al., 2017; Lee et al., 2017; Li et al., 2017c; Qiao et al., 2017; Zeng et al., 2017; Cho et al., 2018; Gong et al., 2018; Nguyen et al., 2018; Wang et al., 2018a; Wang et al., 2018b; Wu et al., 2018; Zhou et al., 2018; Huang et al., 2019a; Luo et al., 2019; Moon et al., 2019; Song et al., 2019; Yuan et al., 2019; Wu et al., 2019) Stability of the insertion through multiple generations Necessary specialized structure (physical and technical).
Plant chemical element transporters (Wu et al., 2017;Kim et al., 2018a; Oda et al., 2018)
Biotic stress resistance (Vo et al., 2018; Yoo et al., 2018; Ma et al., 2019)
Yield and quality (Um et al., 2018; Xiao et al., 2018a; Yang et al., 2018; Wang et al., 2018c; Huang et al., 2019b)
Abiotic stress tolerance (Almadanim et al., 2017; Chen et al., 2018; Kwon et al., 2018; Giri et al., 2018; Li et al., 2018d; Redillas et al., 2019; Tang et al., 2019; Xia et al., 2018; Xiao et al., 2018b; Zhang et al., 2018; Deng et al., 2019; Qin et al., 2019; Zhan et al., 2019; Wang et al., 2019)
Transposon Random sequence insertion 51% (in 4413 families) (Kolesnik et al., 2004) Plant development and metabolism (Ramamoorthy et al., 2018; Ke et al., 2019); Ac/Ds transpose at high frequencies in rice.
Retrotransposon Random sequence insertion 1 insertion each 100-kb (Miyao et al., 2003) Plant development and metabolism (Hori et al., 2016; Mieulet et al., 2016; Chen et al., 2017b) Insertion events are more frequent in genic regions.
Yield and quality (Hirose et al., 2017; Imagawa et al., 2018)
Industrial quality (Nakata et al., 2018)

*From the last 2 years.