Table 1. Primer sequences used to analyse the mRNA levels of genes of interest and reference genes.
Forward (5’-3’) | Reverse (3’-5’) | |
---|---|---|
IL-6 | CCACCTCAAATGGACCACTACTC | TTTTCAGGGCAGAGATTTTGC |
TNF-α | AAAGGACATCATGAGCACTGAAAG | GGGCCCCCTGCCTTCT |
CD68 | CTTTGGGCCAAGTTTCTCTTGT | AAGAGGCCGAGGAGGATCAG |
IL-1β | CGGCAATGAGAATGACCTGT | GCTTCTCCACAGCCACAATG |
Haptoglobin | AGAAAGCAGCCTGTGGAGAT | AGCCAGACACATAACCCACA |
NF-kB | GCTTTGTGACAAGGTGCAGA | ACGATCATCTGTGTCTGGCA |
FGF-21 | GATGATGCCCAGGAGACAGA | AAGTGGAGCGATCCGTACAG |
ATF4 | TGGTCTCAGACAACAGCAAG | AGCTCATCTGGCATGGTTTC |
RPL32 | AGCCATCTACTCGGCGTCA | TCCAATGCCTCTGGGTTTC |