Figure 1.

Fresh and dry weight analysis of developing seeds. Florets were marked at the time of fertilization to track the precise developmental timing. One day after fertilization (DAF), plants were either kept in control conditions or moved to greenhouse with adjusted HNT. Only the marked developing seeds at 4, 7, and 10 days after fertilization were harvested and analyzed. (A) Fresh weight was measured immediately after harvesting. (B) For dry weights, the seeds were subjected to drying for 7 days at 40 ˚C prior to measurements. For fresh and dry weight measurements (in mg), sum of ten developing seeds derived from three plants for the respective time-point and treatment was considered. Data is represented as mean ± standard deviation from three independent replicates. Student’s t-test was used for the statistical analysis (***p < 0.001, **p < 0.01, *p < 0.05). HNT, high night-time temperature.