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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: J Acquir Immune Defic Syndr. 2019 Dec 15;82(5):503–513. doi: 10.1097/QAI.0000000000002185

Figure 4: Differential induction of pro and anti-inflammatory cytokines by recombinant human IL-32α, β, and γ isoforms.

Figure 4:

Purified CD4+ T-cells were stimulated with plate-coated anti-CD3 (1μg/ml) and soluble anti-CD28 (0.5μg/ml) in the presence or absence of IL-32α, IL-32β or IL-32γ (500ng/ml) for 48h. A) IL-10 production by TCR-stimulated cells in the presence of IL-32α, IL-32β or IL-32γ (Left panel, n=22). Middle and right panels: matched pair analysis for IL-10 production by IL-32 isoforms. B) IL-6 production (n=14) and C) IFN-γ production (n=11) as in A. Data were analyzed by the non-parametric Kruskal-Wallis for multiple comparisons (A, B and C left panels) and Wilcoxon for matched pair analysis (A, B and C, middle and right panels).