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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Leuk Res. 2019 Jul 3;84:106180. doi: 10.1016/j.leukres.2019.106180

Figure 4. IL-8 induces chemoresistance which is abrogated using NCI34255.

Figure 4.

(A) The response of KG-1 cells in monoculture or non-adherent KG-1 cells in co-culture to Ara-C was tested and showed that KG-1 cells are significantly less sensitive to Ara-C when cultured with ECs. ** p < 0.01 (B) Co-cultures were treated with Ara-C, NCI34255 or a combination of both. Significant decreases in cell viability was observed when Ara-C alone treated groups were compared to Ara-C + NCI34255 treated groups demonstrating that NCI34255 was able to augment Ara-C and enhance apoptotic responses. NCI34255 alone treated groups did affect cell viability in comparison to vehicle controls. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 (C, D) Analysis of Akt activity in non-adherent KG-1 cells collected from EC co-culture treated with NCI34255 showed significantly decreased levels of phosphorylated Akt in comparison to non-treated controls (C). A similar analysis also showed a significant decrease in phosphorylated 4E-BP1 a downstream target of mTORC1 in the same cell population (D). ** p < 0.01 (E) Bax expression in KG-1 cells isolated from co-cultures exposed to NCI34255 treatment was assessed. The results showed that Bax is cleaved in response to NCI34255 forming the apoptosis enhancing truncated form, p18Bax.