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. Author manuscript; available in PMC: 2019 Nov 15.
Published in final edited form as: Cell Rep. 2019 May 28;27(9):2737–2747.e5. doi: 10.1016/j.celrep.2019.04.114

Figure 3. ERK Regulates Gja1 Transcript Isoforms and Alternative Translation.

Figure 3.

(A) Western blot of cell lysates probed with Cx43 C-terminal antibody to detect full-length Cx43 and internally translated GJA1–20k following treatment with DMSO (Veh) or ERK inhibitor (ERKi) SCH772984 with loading control α-tubulin.

(B) Quantification of bands from (A).

(C–E) qRT-PCR analysis of L (C), M (D), and S (E) UTR isoforms following treatment with Veh (DMSO) or ERK inhibitor. Statistical analysis performed using the Student’s t test (n = 3), *p ≤ 0.05, **p ≤ 0.01.