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. Author manuscript; available in PMC: 2019 Nov 15.
Published in final edited form as: Cell Rep. 2019 May 28;27(9):2737–2747.e5. doi: 10.1016/j.celrep.2019.04.114

Figure 5. Gja1 5′ UTRs Regulate Translation Efficiency.

Figure 5.

(A) Schematic of RNA constructs.

(B–D) Firefly luciferase (Fluc) reporter gene expression normalized to renilla luciferase (Rluc) for L (B), M (C), and S (D) 5′ UTR sequence when capped (m7G), uncapped or A-capped (A), and located internally in a bicistronic construct (dual).

(E–G) Fluc reporter gene expression normalized to Rluc for L (E), M (F), and S (G) m7G-capped 5′ UTR sequences following transfection into cells exposed to TGF-β for 48 h.

Statistical analysis was performed using the Student’s t test (n = 3), ***p ≤ 0.001, ****p ≤ 0.0001.