| Antibodies |
|
|
| rabbit anti-Connexin-43 |
Sigma-Aldrich |
Cat# C6219, RRID:AB_476857 |
| phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb |
Cell Signaling Technology |
Cat# 4511, RRID:AB_2139682 |
| p38 MAPK (D13E1) XP® Rabbit |
Cell Signaling Technology |
Cat# 8690, RRID:AB_10999090 |
| mouse monoclonal anti-alpha-tubulin |
Sigma-Aldrich |
Cat# T6199, RRID:AB_477583 |
| Mouse Anti-Rabbit Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Monoclonal Antibody, Unconjugated, Clone 6C5 |
Fitzgerald Industries International |
Cat# 10R-G109a, RRID:AB_1285808 |
| Mouse monoclonal anti-pan cadherin |
Novus Biologicals |
Novus Cat# NB 100–1907, RRID:AB_530742 |
| Mouse monoclonal anti–N-cadherin |
BD Biosciences |
Cat# 610921, RRID:AB_398236 |
| Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate |
Thermo Fisher Scientific |
Cat# A-11034, RRID:AB_2576217 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 |
Thermo Fisher Scientific |
Cat# A-21245, RRID:AB_2535813 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 |
Thermo Fisher Scientific |
Cat# A-21424, RRID:AB_141780 |
| Goat Anti-Rabbit IgG - H&L Polyclonal antibody, Hrp Conjugated |
Abcam |
Abcam Cat# ab6721, RRID:AB_955447 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Human TGF-β1 |
Humanzyme |
HZ-1011 |
| SB 202190, p38 MAP kinase inhibitor |
Cayman Chemical |
Cat# 10010399, CAS:152121–30-7 |
| SP 600125, JNK inhibitor |
Cayman Chemical |
Cat# 10010466, CAS:129–56-6 |
| SCH 772984, ERK1/2 inhibitor |
Cayman Chemical |
Cat# 19166, CAS:942183–80-4 |
| PureLink DNase |
Thermo Fisher Scientific |
Cat# 12185010 |
| HALT Protease and Phosphatase Inhibitor Cocktail |
Thermo Fisher Scientific |
Cat# 78440 |
| 4X Bolt LDS sample buffer |
Thermo Fisher Scientific |
Cat# B0007 |
| Lipofectamine 3000 reagent |
Thermo Fisher Scientific |
Cat# L3000015 |
| Collagenase, Type 2 |
Worthington Biochemical Corporation |
Cat# LS004176
|
| Insulin-Transferrin-Selenium (ITS -G) (100X) |
Thermo Fisher Scientific |
Cat# 41400045 |
| ARCA M7G cap |
New England BioLabs |
Cat# S1411 |
| G(5′)ppp(5′)A RNA Cap Structure Analog |
New England BioLabs |
Cat# S1406 |
| Dimethyl sulfoxide |
Sigma-Aldrich |
Cat# D2438, CAS:67–68-5 |
| HBSS |
Thermo Fisher Scientific |
Cat# 14175095 |
| DMEM/F-12, GlutaMAX |
Thermo Fisher Scientific |
Cat# 10565018 |
| MycoZap Plus-CL |
Thermo Fisher Scientific |
Cat# VZA-2012 |
| Normal Goat Serum |
Thermo Fisher Scientific |
Cat# 01–6201 |
| DAPI (4′,6-diamidino-2-phenylindole) |
Thermo Fisher Scientific |
Cat# D1306 |
| ProLong Gold Antifade Mountant |
Thermo Fisher Scientific |
Cat# P36934
|
| TRIzol |
Thermo Fisher Scientific |
Cat# 15596026 |
| clarity western ECL substrate |
BioRad |
Cat# 1705060 |
| Critical Commercial Assays |
|
|
| GeneRacer Kit with SuperScript III RT and Zero Blunt TOPO PCR Cloning Kit for Sequencing |
Thermo Fisher Scientific |
Cat# L150202 |
| Dual-Luciferase Reporter Assay System |
Promega |
Cat# E1910 |
| mMESSAGE mMACHINE T7 Transcription Kit |
Thermo Fisher Scientific |
Cat# AM1344 |
| iScript Reverse Transcription Supermix for RT-qPCR |
BioRad |
Cat# 1708840 |
| Purelink RNA Mini Kit |
Thermo Fisher Scientific |
Cat# 12183018A |
| SYBR Select Master Mix for CFX |
Thermo Fisher Scientific |
Cat# 4472942 |
| DC Protein Assay Reagents |
BioRad |
Cat# 5000116 |
| In-fusion HD cloning |
Takara |
Cat# 638909 |
| Papain Dissociation kit |
Worthington Biochemical Corporation |
Cat# LK003153
|
| anti-myelin Microbeads |
Miltenyi Biotec |
Cat# 130096733 |
| anti-Cd11b+ Microbeads |
Miltenyi Biotec |
Cat# 130093634 |
| Experimental Models: Cell Lines |
|
|
| NMuMG cells |
Laboratory of Rik Derynck |
N/A |
| 293 FT cells |
Thermo Fisher Scientific |
Cat# R70007
|
| Human iPSC-derived cardiomyocytes |
Axol Biosciences |
Cat# ax2505 |
| NMuMG Gja1−/− clone6 |
This paper |
N/A |
| 293 FT GJA1−/ − clone 30 |
This paper |
N/A |
| Experimental Models: Organisms/Strains |
|
|
| C57BL/6N |
Charles River Laboratories |
N/A |
| C57BL/6N |
National Institute on Aging |
N/A |
| Oligonucleotides |
|
|
| GJA1 KO guide RNA: AGAAACAAAGAGACAATTCA |
This paper |
N/A |
| GJA1 KO guide RNA: TGGCTCTGCTTGAAGGTCGC |
This paper |
N/A |
| Gja1 KO guide RNA: AAGCCTACTCCACGGCCGGA |
This paper |
N/A |
| Gja1 KO guide RNA: CCACAATCGATTGGCAGCTT |
This paper |
N/A |
| CRISPR Screening primers human: GGTTTTGGAAAGAAGGTTATGGA |
This paper |
N/A |
| CRISPR Screening primers human: ATTACCACCCGCTCATTCAC |
This paper |
N/A |
| CRISPR Screening primers mouse: AGGTGGTGTCCAGAGCCTTA |
This paper |
N/A |
| CRISPR Screening primers mouse: GAGGACTTGGATTCCTGCTCA |
This paper |
N/A |
| Primers for RT-qPCR see Table S1
|
This paper |
N/A |
| Primers for RLM-RACE see Table S2
|
This paper |
N/A |
| Recombinant DNA |
|
|
| pSpCas9(BB)-2A-GFP (PX458) |
Ran et al., 2013 |
Addgene plasmid Cat# 48138 |
| pcDNA3.2/V5-DEST |
Thermo Fisher Scientific |
Cat# 12489019 |
| pcDNA3 RLUC POLIRES FLUC |
Poulin et al., 1998 |
Addgene plasmid Cat# 45642 |
| pcDNA3 RLUC-S-FLUC |
This paper |
N/A |
| pcDNA3 RLUC-M-FLUC |
This paper |
N/A |
| pcDNA3 RLUC-L-FLUC |
This paper |
N/A |
| Software and Algorithms |
|
|
| ImageJ |
Schneider et al., 2012 |
https://imagej.nih.gov/ij/ |
| SnapGene |
GSL Biotech |
https://www.snapgene.com/ |
| RT miner |
Zhao and Fernald 2005 |
http://www.ewindup.info/miner/ |
