Table 1. Quality metric comparison between suggested commercial sequencing quality and results from both HTMGS sequencing chips.
| Chip number | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
|---|---|---|---|---|---|---|---|
| Chip loading (%) | 65 | 82 | 77 | 80 | 80 | 61 | 78 |
| Enrichment (%) | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| Polyclonal (%) | 18 | 45 | 45 | 42 | 44 | 19 | 47 |
| Low quality (%) | 3 | 23 | 22 | 11 | 15 | 4 | 15 |
| Primer Dimer (%) | 1 | 0 | 0 | 1 | 0 | 5 | 0 |
|
Usable reads* (%) |
3,182,960 (79.0) |
2,190,789 (42) |
2,040,115 (42) |
2,550,966 (51) |
2,415,427 (48) |
2,839,445 (73) |
2,206,387 (45) |
| Mapped usable reads (%) | 99.7 | 97 | 97 | 96 | 97 | 98 | 96 |
| Mean Read length (bp) | 126 | 159 | 185 | 182 | 180 | 125 | 147 |
| Total bases (>AQ17) | 374Mb | 303Mb | 292Mb | 371Mb | 355Mb | 331Mb | 253Mb |
*This value is dependent on the overall sequencing quality for the rest of the measurements