Table 2. Commercial kit verses HTMGS method cost comparisons for each major step used to complete Ion Torrent PGM whole mitochondrial sequencing.

HTMGS method	Kit	Cost per sample (AUD)
Long range PCR	GoTaq long PCR Master Mix	$2.45
Cleaning and pooling	Qiaquick spin columns	$1.94
	DNA 12000 Bioanalyser kit	$3.62
	50bp ladder	$0.16
Size selection	Egel (2% size select)	$3.85
Library Preparation	Next fast library prep set for ion torrent	$16.80
	Bioo Scientific barcodes (96)	$3.34
	AMPure XP beads	$7.86
	DNA 1000 Bioanalyser kit	$3.62
	Total cost	$43.64
Commercial kit	Kit	Cost per sample (AUD)
Long range PCR	Expand long range dNTP pack (125)	$2.08
Cleaning and pooling	Qiaquick spin columns (100)	$1.94
	DNA 12000 Bioanalyser kit	$3.62
	50bp ladder	$0.16
Size Selection	Egel (2% size select)	$3.85
Library Preparation	IonXpress 1–96 barcodes	$12.10
	Ion plus fragment library kit	$43.60
	AMPure XP beads	$7.86
	DNA 1000	$3.62
	Total cost	$78.83

All costs shown are current as of November 2018. All materials and prices shown in bold differ from the HTMGS protocol and other reagents were assumed to be comparable.