TABLE I.
Extracted cellular electrical constants (cytoplasm conductivity, S/m; specific membrane capacitance, mF/m2; and conductance, S/m2) of Kc167 cells.
| Isotonic sucrose/glucose | Hypertonic sucrose/glucose | Hypertonic sucrose/glucose | |
|---|---|---|---|
| medium + CaCl2 and MgCl2 | medium (+200 mM sucrose) | medium (+400 mM sucrose) | |
| (360 mOsmol/kg) | (560 mOsmol/kg) | (760 mOsmol/kg) | |
| Cytoplasm conductivity (S/m) | |||
| Kc167 vector control (VC) | 0.152 ± 0.016 (n = 68) | 0.192 ± 0.009a (n = 62) | 0.189 ± 0.012a (n = 61) |
| Nontransfected control | 0.162 ± 0.014 (n = 18) | ||
| VC-dead | 0.032 ± 0.002b (n = 7) | ||
| AfrLEA3m | 0.215 ± 0.018b (n = 72) | 0.172 ± 0.007a,b (n = 63) | 0.139 ± 0.014a,b (n = 58) |
| AfrLEA6 | 0.23 ± 0.0186b (n = 72) | 0.188 ± 0.0163a (n = 61) | 0.126 ± 0.015a,b (n = 57) |
| Specific membrane capacitance (mF/m2) | |||
| Kc167 vector control (VC) | 10 ± 1.6 (n = 68) | 11.4 ± 1.18 (n = 62) | 12.6 ± 1.3 (n = 61) |
| Nontransfected control | 10.5 ± 1.6 (n = 18) | ||
| VC-dead | 2.7 ± 0.06b (n = 7) | ||
| AfrLEA3m | 9.8 ± 1.2 (n = 72) | 14.7 ± 1.6a,b (n = 63) | 14.2 ± 0.8a,b (n = 58) |
| AfrLEA6 | 11.8 ± 1.8 (n = 72) | 12.4 ± 1.53 (n = 61) | 15.0 ± 3.0 (n = 57) |
| Specific membrane conductance (S/m2) | |||
| Kc167 vector control (VC) | 793 ± 63 (n = 68) | 1114 ± 80a (n = 62) | 1129 ± 132a (n = 61) |
| Nontransfected control | 812 ± 38 (n = 18) | ||
| VC-Dead | 503 ± 43b (n = 7) | ||
| AfrLEA3m | 758 ± 75 (n = 72) | 878 ± 73b (n = 63) | 915 ± 73a,b (n = 58) |
| AfrLEA6 | 710 ± 42 (n = 72) | 956 ± 62a,b (n = 61) | 1050 ± 110a (n = 57) |
| Cell diameter (μm) | 10 ± 0.23 (n = 7) | 9.2 ± 0.31 (n = 10) | 8.9 ± 0.21 (n = 10) |
a
Values differ significantly from the corresponding isotonic medium (one-way ANOVA; P < 0.05), total number of trials, n = nbio ( ∼ 5–6) × ntec( ∼ 10–12).
b
Values differ significantly from the corresponding control (one-way ANOVA; P < 0.05), total number of trials, n = nbio ( ∼ 5–6) × ntec( ∼ 10–12).