Table 3.

Number of salt bridges formation in the native and mutant proteins (R74C, S66W, and R245H)

Salt Bridges		Distance between residues of native and mutant proteins (Å)
Residue 1	Residue 2	Native	R245H	R74C	S66W
ND1 HIS A 178	OD2 ASP A 31	2.91	2.89	2.89	2.90
ND1 HIS A 429	OD1 ASP A 426	2.84	2.82	2.82	2.82
ND1 HIS A 429	OD2 ASP A 426	3.92	3.94	3.94	3.95
ND1 HIS A 84	OD1 ASP A 477	3.98	3.99	3.99	3.99
ND1 HIS A 84	OD2 ASP A 477	3.76	3.77	3.78	3.78
NE2 HIS A 181	OD2 ASP A 32	3.96	3.93	3.93	3.93
NE2 HIS A 245	OD2 ASP A 179	–	3.56	–	–
NE2 HIS A 383	OD1 ASP A 440	–	3.99	3.99	4.00
NH1 ARG A 150	OD1 ASP A 179	2.62	2.72	2.74	2.71
NH1 ARG A 169	OD2 ASP A 135	3.98	–	–	–
NH1 ARG A 182	OD1 ASP A 235	3.01	3.03	3.03	3.03
NH1 ARG A 182	OD2 ASP A 235	3.27	3.23	3.22	3.24
NH1 ARG A 282	OD2 ASP A 32	3.08	3.07	3.07	3.08
NH1 ARG A 304	OE1 GLU A 355	3.62	3.58	3.58	3.58
NH2 ARG A 160	OE1 GLU A 256	3.80	3.82	3.82	3.82
NH2 ARG A 169	OD2 ASP A 167	3.28	3.27	3.27	3.27
NH2 ARG A 182	OD1 ASP A 235	3.80	3.87	3.87	3.84
NH2 ARG A 182	OD2 ASP A 235	2.85	2.89	2.89	2.88
NH2 ARG A 245	OD1 ASP A 179	2.98	–	2.97	2.97
NH2 ARG A 245	OD2 ASP A 179	3.77	–	3.83	3.81
NH2 ARG A 282	OD2 ASP A 399	2.59	2.75	2.75	2.71
NH2 ARG A 304	OE1 GLU A 355	2.75	2.76	2.76	2.75
NH2 ARG A 377	OD1 ASP A 477	2.90	2.94	2.94	2.93
NH2 ARG A 377	OD2 ASP A 477	3.27	3.37	3.37	3.35
NH2 ARG A 414	OD1 ASP A 410	3.93	3.93	3.93	3.93
NH2 ARG A 435	OD1 ASP A 484	3.41	3.36	3.36	3.38
NH2 ARG A 456	OD2 ASP A 454	2.73	2.76	2.76	2.75
NH2 ARG A 74	OD1 ASP A 31	2.93	2.97	–	2.96
NH2 ARG A 74	OD2 ASP A 273	3.14	3.12	–	3.12
NH2 ARG A 74	OD2 ASP A 31	3.86	3.92	–	3.91
NZ LYS A 123	OD1 ASP A 31	3.22	3.27	3.27	3.26
NZ LYS A 123	OD2 ASP A 31	2.86	2.91	2.91	2.90
NZ LYS A 124	OE1 GLU A 129	2.96	2.98	2.97	2.98
NZ LYS A 156	OD1 ASP A 209	3.60	3.61	3.61	3.60
NZ LYS A 156	OD2 ASP A 209	2.90	2.92	2.92	2.91
Total no. of salt bridges		33	32	30	33