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. 2019 Nov 15;9:16909. doi: 10.1038/s41598-019-53422-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Corpus luteum, bold arrow mark macrophages; (B) Graafian follicle, bold arrow marks granulosa cells. IS PCR for the detection of CPV2, NBT chromogen and counterstaining with Fast Green; (C) Ileum with low amount of CPV2 antigen, ABC chromogen and counterstaining with Heamtoxylin, 200x; (D) Lymph node with low amount of CPV2 antigen is noticeable in a follicle, IHC to detect CPV2, ABC chromogen and counterstained with Heamtoxylin, 400x.