Table 2.
Pro-inflammatory markers, changes in mRNA and protein levels, and correlation between mRNA and protein expressions.
| Sex | Endpoint | Marker | Region | Analysis | Post-hoc compared to VV | Protein-mRNA | |||
|---|---|---|---|---|---|---|---|---|---|
| Main effect: postnatal | Interaction | ||||||||
| Statistic (df) | P value | Statistic (df) | P value | ||||||
| Male | mRNA | TNFα | mPFC | F(1,16) = 228.87 | <0.0001 | F(1,16) = 4.19 | ns | PV, VL, PL | |
| AMY | F(1,16) = 0.12 | ns | F(1,16) = 2.09 | ns | |||||
| HP | F(1,16) = 84.02 | <0.0001 | F(1,16) = 11.11 | 0.0042 | VL, PL | ||||
| TM | F(1,16) = 208.74 | <0.0001 | F(1,16) = 11.00 | 0.0044 | PV, VL, PL | ||||
| iNOS | mPFC | F(1,16) = 329.51 | <0.0001 | F(1,16) = 172.74 | <0.0001 | PV, VL, PL | |||
| AMY | F(1,16) = 3.43 | ns | F(1,16) = 3.03 | ns | |||||
| HP | F(1,16) = 36.01 | <0.0001 | F(1,16) = 64.70 | <0.0001 | PL | ||||
| TM | F(1,16) = 161.51 | <0.0001 | F(1,16) = 29.03 | <0.0001 | VL, PL | ||||
| IL-6 | mPFC | F(1,16) = 476.19 | <0.0001 | F(1,16) = 241.79 | <0.0001 | PV, VL, PL | |||
| AMY | F(1,16) = 331.12 | <0.0001 | F(1,16) = 185.11 | <0.0001 | PV, VL, PL | ||||
| HP | F(1,16) = 0.22 | ns | F(1,16) = 0.03 | ns | |||||
| TM | F(1,16) = 4761.16 | <0.0001 | F(1,16) = 3270.32 | <0.0001 | VL, PL | ||||
| IL-1β | mPFC | F(1,16) = 742.96 | <0.0001 | F(1,16) = 389.32 | <0.0001 | VL, PL | |||
| AMY | F(1,16) = 227.53 | <0.0001 | F(1,16) = 203.69 | <0.0001 | PL | ||||
| HP | F(1,16) = 0.90 | ns | F(1,16) = 1.66 | ns | |||||
| TM | F(1,16) = 219.60 | <0.0001 | F(1,16) = 217.58 | <0.0001 | PL | ||||
| Female | mRNA | TNFα | mPFC | F(1,16) = 126.28 | <0.0001 | F(1,16) = 0.36 | ns | PV, VL, PL | |
| AMY | F(1,16) = 1.37 | ns | F(1,16) = 8.96 | 0.0086 | |||||
| HP | F(1,16) = 6.64 | 0.0203 | F(1,16) = 435.01 | <0.0001 | PV, VL, PL | ||||
| TM | F(1,16) = 126.54 | <0.0001 | F(1,16) = 407.69 | <0.0001 | PV, VL | ||||
| iNOS | mPFC | F(1,16) = 101.98 | <0.0001 | F(1,16) = 0.36 | ns | VL, PL | |||
| AMY | F(1,16) = 88.52 | <0.0001 | F(1,16) = 154.85 | <0.0001 | PV, VL | ||||
| HP | F(1,16) = 47.78 | <0.0001 | F(1,16) = 278.79 | <0.0001 | PV, VL | ||||
| TM | F(1,16) = 0.36 | ns | F(1,16) = 81.59 | <0.0001 | PV, VL | ||||
| IL-6 | mPFC | F(1,16) = 217.47 | <0.0001 | F(1,16) = 25.92 | 0.0001 | VL, PL | |||
| AMY | F(1,16) = 224.38 | <0.0001 | F(1,16) = 271.55 | <0.0001 | PV, VL, PL | ||||
| HP | F(1,16) = 5.06 | 0.0389 | F(1,16) = 41.85 | <0.0001 | PV | ||||
| TM | F(1,16) = 6.02 | 0.026 | F(1,16) = 149.25 | <0.0001 | PV, VL | ||||
| IL-1β | mPFC | F(1,16) = 14.05 | 0.0018 | F(1,16) = 49.06 | <0.0001 | PV, VL, PL | |||
| AMY | F(1,16) = 2.29 | ns | F(1,16) = 248.65 | <0.0001 | PV, VL, PL | ||||
| HP | F(1,16) = 9.36 | 0.0075 | F(1,16) = 112.82 | <0.0001 | PV, VL, PL | ||||
| TM | F(1,16) = 15.34 | 0.0012 | F(1,16) = 9.03 | 0.0084 | VL | ||||
| Male | protein | IL-6 | mPFC | F(1,8) = 1.70 | ns | F(1,8) = 1.47 | ns | R2 = 0.0458, ns | |
| AMY | F(1,8) = 7.76 | 0.0237 | F(1,8) = 0.03 | ns | PL | R2 = 0.2755, ns | |||
| HP | F(1,8) = 5.97 | 0.0403 | F(1,8) = 0.34 | ns | R2 = 0.001, ns | ||||
| TM | F(1,8) = 116.22 | <0.0001 | F(1,8) = 3.72 | ns | VL, PL | R2 = 0.7313, P = 0.0004 | |||
| IL-1β | mPFC | F(1,8) = 289.30 | <0.0001 | F(1,8) = 68.24 | <0.0001 | VL, PL | R2 = 0.9592, P < 0.0001 | ||
| AMY | F(1,8) = 349.76 | <0.0001 | F(1,8) = 135.26 | <0.0001 | VL, PL | R2 = 0.9343, P < 0.0001 | |||
| HP | F(1,8) = 0.62 | ns | F(1,8) = 0.90 | ns | R2 = 0.0179, ns | ||||
| TM | F(1,8) = 76.01 | <0.0001 | F(1,8) = 77.07 | <0.0001 | PL | R2 = 0.9541, P < 0.0001 | |||
| Female | protein | IL-6 | mPFC | F(1,8) = 1.36 | ns | F(1,8) = 0.29 | ns | R2 = 0.3689, P = 0.0362 | |
| AMY | F(1,8) = 21.02 | 0.0018 | F(1,8) = 13.50 | 0.0063 | PV, VL, PL | R2 = 0.4773, P = 0.0129 | |||
| HP | F(1,8) = 17.71 | 0.003 | F(1,8) = 0.01 | ns | R2 = 0.062, ns | ||||
| TM | F(1,8) = 1.95 | ns | F(1,8) = 0.14 | ns | R2 = 0.016, ns | ||||
| IL-1β | mPFC | F(1,8) = 0.11 | ns | F(1,8) = 9.41 | 0.0154 | PV | R2 = 0.4386, P = 0.019 | ||
| AMY | F(1,8) = 0.00 | ns | F(1,8) = 23.59 | 0.0013 | R2 = 0.7228, P = 0.0005 | ||||
| HP | F(1,8) = 1.24 | ns | F(1,8) = 1.99 | ns | R2 = 0.2616, ns | ||||
| TM | F(1,8) = 12.03 | 0.0085 | F(1,8) = 1.67 | ns | R2 = 0.4137, P = 0.0241 | ||||
Statistical analysis of pro-inflammatory mRNA and protein markers by brain region for males and females. ns, not significant; VV, Vehicle-Vehicle; PV, Poly I:C-Vehicle; VL, Vehicle-LPS; PL, Poly I:C-LPS.