Table 3.
Neuroinflammation markers, changes in mRNA and protein levels, and correlation between mRNA and protein expressions.
| Sex | Endpoint | Marker | Region | Analysis | Post-hoc compared to VV | Protein-mRNA | |||
|---|---|---|---|---|---|---|---|---|---|
| Main effect: postnatal | Interaction | ||||||||
| Statistic (df) | P value | Statistic (df) | P value | ||||||
| Male | mRNA | Iba-1 | mPFC | F(1,16) = 230.33 | <0.0001 | F(1,16) = 8.62 | 0.0097 | VL, PL | |
| AMY | F(1,16) = 165.08 | <0.0001 | F(1,16) = 56.41 | <0.0001 | VL, PL | ||||
| HP | F(1,16) = 593.80 | <0.0001 | F(1,16) = 38.33 | <0.0001 | PV, VL, PL | ||||
| TM | F(1,16) = 232.03 | <0.0001 | F(1,16) = 1.43 | ns | VL, PL | ||||
| GFAP | mPFC | F(1,16) = 474.33 | <0.0001 | F(1,16) = 59.83 | <0.0001 | VL, PL | |||
| AMY | F(1,16) = 657.53 | <0.0001 | F(1,16) = 379.92 | <0.0001 | PV, VL, PL | ||||
| HP | F(1,16) = 917.53 | <0.0001 | F(1,16) = 13.15 | 0.0023 | VL, PL | ||||
| TM | F(1,16) = 947.50 | <0.0001 | F(1,16) = 0.01 | ns | VL, PL | ||||
| TSPO | mPFC | F(1,16) = 868.23 | <0.0001 | F(1,16) = 81.84 | <0.0001 | VL, PL | |||
| AMY | F(1,16) = 733.73 | <0.0001 | F(1,16) = 364.60 | <0.0001 | PV, VL, PL | ||||
| HP | F(1,16) = 664.81 | <0.0001 | F(1,16) = 30.96 | <0.0001 | PV, VL, PL | ||||
| TM | F(1,16) = 470.88 | <0.0001 | F(1,16) = 363.57 | <0.0001 | PL | ||||
| Female | mRNA | Iba-1 | mPFC | F(1,16) = 227.77 | <0.0001 | F(1,16) = 0.76 | ns | PV, VL, PL | |
| AMY | F(1,16) = 70.97 | <0.0001 | F(1,16) = 10.80 | 0.0046 | PV, VL, PL | ||||
| HP | F(1,16) = 4.09 | ns | F(1,16) = 23.88 | 0.0002 | PV, VL | ||||
| TM | F(1,16) = 50.14 | <0.0001 | F(1,16) = 0.92 | ns | VL, PL | ||||
| GFAP | mPFC | F(1,16) = 299.84 | <0.0001 | F(1,16) = 113.53 | <0.0001 | PV, VL, PL | |||
| AMY | F(1,16) = 16.11 | 0.001 | F(1,16) = 0.14 | ns | |||||
| HP | F(1,16) = 1.72 | ns | F(1,16) = 0.02 | ns | |||||
| TM | F(1,16) = 0.08 | ns | F(1,16) = 0.07 | ns | |||||
| TSPO | mPFC | F(1,16) = 59.59 | <0.0001 | F(1,16) = 3.23 | ns | PV, VL, PL | |||
| AMY | F(1,16) = 122.66 | <0.0001 | F(1,16) = 97.23 | <0.0001 | PV, VL, PL | ||||
| HP | F(1,16) = 7.16 | 0.0165 | F(1,16) = 130.88 | <0.0001 | PV, VL, PL | ||||
| TM | F(1,16) = 0.01 | ns | F(1,16) = 2.14 | ns | PL | ||||
| Male | protein | Iba-1 | mPFC | F(1,8) = 165.92 | <0.0001 | F(1,8) = 8.74 | 0.0182 | PV, VL, PL | R2 = 0.5748, P = 0.0043 |
| AMY | F(1,8) = 40.10 | 0.0002 | F(1,8) = 34.41 | 0.0004 | PV, VL, PL | R2 = 0.7034, P = 0.0007 | |||
| HP | F(1,8) = 17.97 | 0.0028 | F(1,8) = 5.80 | 0.0427 | VL | R2 = 0.5466, P = 0.006 | |||
| TM | F(1,8) = 108.06 | <0.0001 | F(1,8) = 0.09 | ns | VL, PL | R2 = 0.5818, P = 0.0039 | |||
| GFAP | mPFC | F(1,8) = 37.91 | 0.0003 | F(1,8) = 6.12 | 0.0385 | PV, VL, PL | R2 = 0.3761, P = 0.034 | ||
| AMY | F(1,8) = 54.46 | <0.0001 | F(1,8) = 0.02 | ns | VL, PL | R2 = 0.4205, P = 0.0226 | |||
| HP | F(1,8) = 53.09 | <0.0001 | F(1,8) = 8.80 | 0.018 | PL | R2 = 0.7659, P = 0.0002 | |||
| TM | F(1,8) = 65.20 | <0.0001 | F(1,8) = 28.48 | 0.0007 | PV, VL, PL | R2 = 0.7761, P = 0.0002 | |||
| TSPO | mPFC | F(1,8) = 201.75 | <0.0001 | F(1,8) = 1.00 | ns | VL, PL | R2 = 0.6421, P = 0.0017 | ||
| AMY | F(1,8) = 62.67 | <0.0001 | F(1,8) = 7.66 | 0.0244 | VL, PL | R2 = 0.6699, P = 0.0011 | |||
| HP | F(1,8) = 14.64 | 0.005 | F(1,8) = 0.56 | ns | R2 = 0.2903, ns | ||||
| TM | F(1,8) = 105.84 | <0.0001 | F(1,8) = 3.01 | ns | VL, PL | R2 = 0.7567, P = 0.0002 | |||
| Female | protein | Iba-1 | mPFC | F(1,8) = 30.53 | 0.0006 | F(1,8) = 0.02 | ns | VL, PL | R2 = 0.3553, P = 0.0408 |
| AMY | F(1,8) = 50.77 | <0.0001 | F(1,8) = 0.90 | ns | VL, PL | R2 = 0.3117, ns | |||
| HP | F(1,8) = 0.07 | ns | F(1,8) = 6.09 | 0.0389 | R2 = 0.0375, ns | ||||
| TM | F(1,8) = 67.45 | <0.0001 | F(1,8) = 0.78 | ns | VL, PL | R2 = 0.6582, P = 0.0014 | |||
| GFAP | mPFC | F(1,8) = 42.48 | 0.0002 | F(1,8) = 33.15 | 0.0004 | PL | R2 = 0.2036, ns | ||
| AMY | F(1,8) = 8.95 | 0.0173 | F(1,8) = 0.25 | ns | R2 = 0.334, P = 0.0491 | ||||
| HP | F(1,8) = 246.59 | <0.0001 | F(1,8) = 5.03 | ns | VL, PL | R2 = 0.0032, ns | |||
| TM | F(1,8) = 0.00 | ns | F(1,8) = 0.04 | ns | R2 = 0.0032, ns | ||||
| TSPO | mPFC | F(1,8) = 2.07 | ns | F(1,8) = 0.66 | ns | R2 = 0.1452, ns | |||
| AMY | F(1,8) = 2.61 | ns | F(1,8) = 2.08 | ns | R2 = 0.2999, ns | ||||
| HP | F(1,8) = 0.14 | ns | F(1,8) = 6.04 | 0.0394 | R2 = 0.4028, P = 0.0266 | ||||
| TM | F(1,8) = 0.10 | ns | F(1,8) = 0.24 | ns | R2 = 0.1575, ns | ||||
Statistical analysis of neuroinflammatory mRNA and protein markers by brain region for males and females. ns, not significant; VV, Vehicle-Vehicle; PV, Poly I:C-Vehicle; VL, Vehicle-LPS; PL, Poly I:C-LPS.