Table 4.
Anti-inflammatory markers, changes in mRNA and protein levels, and correlation between mRNA and protein expressions.
| Sex | Endpoint | Marker | Region | Analysis | Post-hoc compared to VV | Protein-mRNA | |||
|---|---|---|---|---|---|---|---|---|---|
| Main effect: postnatal | Interaction | ||||||||
| Statistic (df) | P value | Statistic (df) | P value | ||||||
| Male | mRNA | IL-10 | mPFC | F(1,16) = 79.79 | <0.0001 | F(1,16) = 2.86 | ns | PV, VL, PL | |
| AMY | F(1,16) = 70.56 | <0.0001 | F(1,16) = 0.05 | ns | VL, PL | ||||
| HP | F(1,16) = 55.94 | <0.0001 | F(1,16) = 1.16 | ns | VL, PL | ||||
| TM | F(1,16) = 161.34 | <0.0001 | F(1,16) = 38.84 | <0.0001 | VL, PL | ||||
| TGF-β1 | mPFC | F(1,16) = 141.11 | <0.0001 | F(1,16) = 0.00 | ns | PV, VL, PL | |||
| AMY | F(1,16) = 77.42 | <0.0001 | F(1,16) = 3.92 | ns | VL, PL | ||||
| HP | F(1,16) = 123.40 | <0.0001 | F(1,16) = 3.23 | ns | VL, PL | ||||
| TM | F(1,16) = 50.82 | <0.0001 | F(1,16) = 0.02 | ns | VL, PL | ||||
| Female | mRNA | IL-10 | mPFC | F(1,16) = 3.69 | ns | F(1,16) = 202.28 | <0.0001 | PV, VL, PL | |
| AMY | F(1,16) = 88.10 | <0.0001 | F(1,16) = 300.38 | <0.0001 | PV, VL, PL | ||||
| HP | F(1,16) = 1.89 | ns | F(1,16) = 1510.63 | <0.0001 | PV, VL, PL | ||||
| TM | F(1,16) = 643.83 | <0.0001 | F(1,16) = 8.26 | 0.011 | PV, VL, PL | ||||
| TGF-β1 | mPFC | F(1,16) = 58.11 | <0.0001 | F(1,16) = 0.01 | ns | PV, VL, PL | |||
| AMY | F(1,16) = 33.59 | <0.0001 | F(1,16) = 55.28 | <0.0001 | PV, VL, PL | ||||
| HP | F(1,16) = 17.42 | 0.0007 | F(1,16) = 165.53 | <0.0001 | PV, VL | ||||
| TM | F(1,16) = 187.92 | <0.0001 | F(1,16) = 19.47 | 0.0004 | PV, VL, PL | ||||
| Male | protein | IL-10 | mPFC | F(1,8) = 82.58 | <0.0001 | F(1,8) = 17.11 | 0.0033 | PV, VL, PL | R2 = 0.7171, P = 0.0005 |
| AMY | F(1,8) = 30.02 | 0.0006 | F(1,8) = 5.63 | 0.045 | VL, PL | R2 = 0.6021, P = 0.003 | |||
| HP | F(1,8) = 16.14 | 0.0039 | F(1,8) = 7.16 | 0.0281 | PV, VL, PL | R2 = 0.6418, P = 0.0017 | |||
| TM | F(1,8) = 19.42 | 0.0023 | F(1,8) = 10.54 | 0.0118 | PL | R2 = 0.433, P = 0.02 | |||
| TGF-β1 | mPFC | F(1,8) = 25.87 | 0.0009 | F(1,8) = 0.75 | ns | PL | R2 = 0.548, P = 0.0059 | ||
| AMY | F(1,8) = 36.91 | 0.0003 | f(1,8) = 0.34 | ns | VL, PL | R2 = 0.6755, P = 0.001 | |||
| HP | F(1,8) = 40.91 | 0.0002 | F(1,8) = 0.00 | ns | VL, PL | R2 = 0.2955, ns | |||
| TM | F(1,8) = 24.83 | 0.0011 | F(1,8) = 2.30 | ns | VL, PL | R2 = 0.3307, P = 0.0505 | |||
| Female | protein | IL-10 | mPFC | F(1,8) = 6.20 | 0.0376 | F(1,8) = 16.49 | 0.0036 | PV, VL | R2 = 0.4854, P = 0.0118 |
| AMY | F(1,8) = 1.52 | ns | F(1,8) = 9.75 | 0.0142 | R2 = 0.3111, ns | ||||
| HP | F(1,8) = 0.02 | ns | F(1,8) = 24.18 | 0.0012 | PV, VL | R2 = 0.4811, P = 0.0124 | |||
| TM | F(1,8) = 42.89 | 0.0002 | F(1,8) = 2.33 | ns | VL, PL | R2 = 0.6289, P = 0.0021 | |||
| TGF-β1 | mPFC | F(1,8) = 6.95 | 0.0299 | F(1,8) = 0.05 | ns | PL | R2 = 0.5465, P = 0.006 | ||
| AMY | F(1,8) = 34.02 | 0.0004 | F(1,8) = 19.17 | 0.0024 | VL | R2 = 0.2629, ns | |||
| HP | F(1,8) = 1.15 | ns | F(1,8) = 26.56 | 0.0009 | PV | R2 = 0.4865, P = 0.0141 | |||
| TM | F(1,8) = 55.57 | <0.0001 | F(1,8) = 4.14 | ns | VL, PL | R2 = 0.7558, P = 0.0002 | |||
Statistical analysis of anti-inflammatory mRNA and protein markers by brain region for males and females. ns, not significant; VV, Vehicle-Vehicle; PV, Poly I:C-Vehicle; VL, Vehicle-LPS; PL, Poly I:C-LPS.