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. 2019 Nov 15;9:16944. doi: 10.1038/s41598-019-53414-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of hiPSC-derived astrocytes. (a) The hiPSC-derived astrocytes expressed several astrocyte-specific genes, including GFAP, VIM, S100B, EAAT1 and EAAT2. The data are presented as the mean ± s.e.m. (n = 2 with three technical replicates; the data are representative of two experiments). (b) Immunocytochemical staining was used to confirm the expression of the major astrocyte markers GFAP, S100β, GS, Aldh1L1, EAAT1 and EAAT2.