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. 2019 Nov 15;9:16922. doi: 10.1038/s41598-019-53097-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Knockdown of PTBP1 expression enhances tumor growth. CL1-5 cells transfected with PTBP1 shRNA or control vector were implanted subcutaneously, at 1 × 10⁶ cells/mouse, into the flanks of nude mice. Tumors were observed and volumes measured up to 7 weeks post implantation. Bars represent SD. *p < 0.05. (B) Representative tumor nodules excised at sacrifice. (C) CL1-5 xenograft tumor weights. At week 7 after implantation, mice were euthanized and tumor nodules were excised, weighed, and presented as mean ± SD. *p < 0.05.